Analysis Note

ControlARPE-19 cell lysate

Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinesin-like protein KIFC3 in HUVEC and HeLa cells.

Detect Kinesin-like protein KIFC3 using this Anti-Kinesin-like protein KIFC3 Antibody validated for use in Western Blotting, Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Kinesin-like protein (KIFC3) is a member of the kinesin superfamily. It is a ubiquitous protein, with relatively high levels of expression in the kidney, testis, adrenal glands, and ovary. It is a minus-end motor protein that uses energy derived from ATP hydrolysis to drive the transport of protein cargo along microtubules. Kinesins play important roles in mediating many cellular processes, such as cell division, protein trafficking, and organelle transport.

Immunogen

KLH-conjugated linear peptide corresponding to human Kinesin-like protein KIFC3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in ARPE-19 cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected Kinesin-like protein KIFC3 in 10 µg of ARPE-19 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~113 kDa observed. The calculated molecular weight is 93 kDa, however Kinesin-like protein KIFC3 has been shown as a ~113 kDa band in western blots (Noda, Y., et al. (2001). J Cell Biol. 155(1):77-88.). An uncharacterized band may appear at ~16 kDa in some lysates.